A Spe-lc-ms/ms Method for Veterinary Antibiotic Analysis in Swine Manure
نویسندگان
چکیده
Veterinary antibiotics evaluation in environmental samples is a very important subject principally because of the development of antibacterial resistance. In this work two SPE-LC-MS/MS methods (acid and basic) were compared and adapted for amoxicillin, lincomycin and tiamulin antibiotics analysis in swine manure. The results showed that the basic method is more suitable for the simultaneous determination of the selected antibiotics with recoveries of 52.1 ± 7.7% for amoxicillin, 64.6 ± 1.6% for lincomycin and 74.7 ± 9.5% for tiamulin.
منابع مشابه
High-throughput Screening Method for Multiple Classes of Antibiotics in Milk Using Automated Sample Preparation and LC-MS/MS
Introduction Veterinary drugs are widely used to prevent the outbreak of disease in livestock and are commonly administered as feed additives or in drinking water. In addition, veterinary drugs are given to treat diseases, for drying-off purposes, or to prevent losses during transportation. Many countries, such as the United States and those in the European Union, have set maximum residue limit...
متن کاملValidation of the analysis method of 25 pesticide residues in rice using LC-MS/MS device (liquid chromatography-mass spectrometry)
Introduction: In recent years, due to the increase in the use of pesticides in agricultural products and its entry into the human food chain, there has been an increase in concerns about the health of food among people. Therefore, the purpose of conducting this research is to validate the analysis method. 25 pesticide residues in rice using LC-MS/MS. Methods: In this research, a simultaneous an...
متن کاملAutomated solid-phase extraction-liquid chromatography-tandem mass spectrometry analysis of 6-acetylmorphine in human urine specimens: application for a high-throughput urine analysis laboratory.
An automated solid-phase extraction-liquid chromatography- tandem mass spectrometry (SPE-LC-MS-MS) method using the Spark Holland Symbiosis Pharma SPE-LC coupled to a Waters Quattro Micro MS-MS was developed for the analysis of 6-acetylmorphine (6-AM) in human urine specimens. The method was linear (R² = 0.9983) to 100 ng/mL, with no carryover at 200 ng/mL. Limits of quantification and detectio...
متن کاملEnzyme-linked immunosorbent assay for ultratrace determination of antibiotics in aqueous samples.
Two commercially available enzyme-linked immunosorbent assay (ELISA) kits that are commonly used for tylosin or tetracycline residues in meat and milk were adapted for ultratrace analysis of these antibiotics in surface and ground waters. These two antibiotics are commonly fed to swine, turkeys, and cattle at subtherapeutic doses for growth promotion purposes. Both ELISA techniques were found t...
متن کاملMethadone Extraction by Modified QuEChERS and Liquid-Liquid Extraction from Post-Mortem Urine by GC-MS
QuEChERS stands for Quick, Easy, Cheap, Effective, Rugged, and Safe. This method is widely used to determine pesticides and was first introduced in 2003. Anastassiades and colleagues first introduced this method in 2003 to extract veterinary drugs from animal tissue [1]. Toxicology samples in forensic medicine are usually analyzed by Liquid-Liquid extraction (LLE) and solid phase extraction (SP...
متن کامل